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BD Influxtm Item #1301
BD Influxtm: 4-laser, 12-color sorter with Spigot v6.1.4 XP workstation (upgradable to Windows 7 FACS Sortware), computerized cell deposition unit (CCDU), sample tube cooling, HEPA filtered enclosure plus filter unit for evacuation of sort chamber, 2-way/3-way/4-way sorting, 7 liter sheath and waste containers, 4 nozzles (70,100,140, 200 micron) and JUN-AIR compressor. Less than 100 hours use. Custom configurations available.
 
Workstation: Spigot XP (upgradable to BD FACS Sortware) with Intel Core2 Duo, 2.40 GHz, 2 USB ports, 1GB memory, 2 x 80GB hard drives, 16x CD/DVD +/- RW and 20-inch LCD monitor.
Lasers: 200mW Coherent 488 nm, 50mW Melles Griot 405nm, 75mW Melles Griot 561nm, 45mW Melles Griot 642nm. Lasers can easily be switched to change laser sequence.
HEPA Enclosure: Includes filter unit for aerosol evacuation of sort chamber.
Fluorescence Sensitivity: <125 MESF-FITC and MESF-PE.
Coefficient of variation (CV): <3.0% for DNA stained with PI.
Nozzles: 70,100, 140 and 200 micron.
Sorting modes: Four-way: microtubes and 12x75mm; Three-way: One 50 ml and two 12x75 mm tubes; Two-way: microtubes, 12x75 mm, 15 ml and 50 ml tubes.
Optics: Emission light collected through microscope objective at 90 degrees and focused on five spatially separated mirror pinholes. Modular detector blocks allow for user-defined detection configuration. Simultaneous video observation of the stream, pinholes and laser intercepts allows for fast and intuitive alignment. Regular forward scatter is detected using 75 and 50-mm lenses, an aperture and a photomultiplier. Small particle option can be added. Side scatter is collected through the 90 degree collection lens and measured with a photomultiplier.
Fluorescence Compensation: 16 x 16 digital compensation matrix (DSP). Compensated parameters are being added to the bus as separate parameters.
Purity and yield: At 60 psi and 100 kHz with an average threshold rate of 25,000 events per second for a four-way sort results in a purity of 98% and a yield > 80% of Poisson's expected yield for all four populations..
Maximum acquisition rate: System dead time is 5 microseconds with a maximum throughput rate of 200,000 events/sec independent of the number of parameters.
ACDU: Allows for slide and plate sorting into 6, 24, 48, 96, and 384-well plates, slides and user-defined collection devices. Sort Collection Cooling Option: Haake water recirculator bath for cooling of tubes.
Sort Collection Cooling Option: Haake water recirculator bath for cooling of tubes.
Air and vacuum supply: JUN-AIR compressor is provided that provides the required 90 psi. Vacuum between 5 and 15 inches Hg is required.
Fluidics System: Autoclavable 7-L sheath and waste containers, equipped with pressure and vacuum readout. Sheath, sample and boost pressure can be individually adjusted. A sample flow fine adjustment is provided for precise regulation of sample flow. Purge, pulse, rinse and run buttons are provided for quick stream startup and bubble removal. The fluidics path can be exchanged including the nozzle asssembly. There are no in-line valves. Only pinch valves are used. The sample line can also be exchanged. Accepts 12 x 75 mm polypropylene tubes.
Power: Two dedicated 120-volt, 50/60-Hz, 15-A lines.
Influx dimensions: 50.4" w x 39.4" d x 55.1" h.
Influx dimensions/weight with HEPA-filtered enclosure: 50.4" w x 49.2" d x 85.8" h / 485 lb.
Manufacture date: 2008
 

Laser and other modifications that better meet the buyer's requirements can be quoted separately. Please inquire.

 
This Influxtm is currently in like-new condition but will be confirmed to meet all original BD specifications. We will perform instrument decontamination and flow cell cleaning procedures. Provide any required restoration of exterior and interior components. Replace filters, fluidics tubing and connectors as required. Clean and inspect sheath and waste containers. Set sample pressure to specifications. Confirm all lasers meet specified wattage. Adjust sample flow rates for correct settings. Clean and align optics for optimum CVs using Redi-Check and Calibrite beads. Confirm sorting meets BD purity and recovery specs. Print all results to be included with shipment and/or faxed to successful bidder for approval.
 
Installation by a qualified service representative quoted separately. The price will include all travel expenses and labor required to confirm proper operation at the customer's facility utilizing standard calibration procedures. Any additional time required to correct malfunctions shall be included in the quoted installation fee.
 
Instrument and/or applications training by a qualified representative are available for an additional fee. The quoted price includes travel and perdiem expenses when training is performed during an installation visit. Otherwise additional reimbursement for travel and perdiem expenses may be required.
 
Custom warranty or service contracts can be quoted separately. All components are covered unless failure is due to operator negligence. In addition, we offer unlimited telephone support for all of the products sold through Spectron Corporation regardless of warranty.
 
Shipping costs will be quoted separately. If the purchaser becomes the shipping agent for one of the products located in the State of Washington they could be subject to paying Washington State sales tax unless exempt. No such obligation exists if Spectron Corporation is the designated shipper to destinations outside the State of Washington. This equipment shall be packed in a sturdy double-walled box surrounded inside by 2" foam sheets on all sides. All components shall be individually protected within the same container. The box will be taped, strapped and attached to a pallet with additional strapping. Shipping will be arranged through a qualified airfreight carrier with full insurance.
 
Most established institutions will be approved for net-30 terms or other payment plans. Leasing or rental programs may also be available for qualified buyers with various options for an eventual purchase. Washington State customers or others arranging shipment or picking up products themselves may be subject to Washington State sales tax unless exempt. Spectron Corporation will inform prospective buyers of any such circumstance that could result in a tax liability. Otherwise, buyers would only be subject to applicable use taxes that they might be required to pay directly to their respective state.
 
Spectron Corporation would appreciate the opportunity to bid on a service contract covering our products as well as other flow cytometers located at your facility. Please inquire into any modifications to the listed products that would better suit your requirements. We strive for complete satisfaction.
 

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